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Buffered Listeria Enrichment Broth

Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp. A modification of Listeria Enrichment Broth developed by Lovett, this media was designed to improve the enrichment of Listeria spp. with the addition of Disodium Phosphate therby increasing the buffering capacity. Listeria spp., found in soil, sewage, and river water in addition to unprocessed foods, are Gram- positive, short and motile rods. Cyclohexmide in this formulation inhibits the growth of saprophytic fungi while Nalidixic Acid is used to inhibit the growth of Gram- negative organisms.