Lauryl Sulfate Broth (aka Lauryl Tryptose Broth)

Lauryl Sulfate Broth, otherwise known as Lauryl Tryptose Broth, is used for the detection of coliforms in water and wastewater. Coliforms are often used as indicators of fecal contamination in water and bacterial contamination in food products. Lactose is added to detect lactose fermentation by coliforms while the Sodium Lauryl Sulfate acts as a selective agent against non-coliform organisms. Sodium Chloride is used to maintain the osmotic balance of the medium while the Potassium Phosphates act as buffering agents.