Plasmid Midi Kit
Isolate up to 200 ug of plasmid DNA in 60 minutes.
E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 ug of plasmid DNA in less than 60 minutes. This kit combines HiBind membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high quality plasmid DNA. DNase/RNase Free HiBind® DNA midicolumns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli Strain, and conditions of growth, 50ml of an overnight culture in LB medium will typically produce 200 ug of high-copy number plasmid DNA. When working with low-copy number plasmids one can increase the starting culture volume up to 100 ml. High-quality DNA recovered is ready for immediate using in routine molecular biology applications such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.